‏660.00 ₪

VETERINARY INFECTION BIOLOGY: MOLECULAR DIAGNOSTICS AND HIGH-THROUGHPUT STRATEGIES

‏660.00 ₪

ISBN13

9781493920037

יצא לאור ב

New York, NY

מהדורה

2015 ed.

עמודים

536

פורמט

Hardback

תאריך יציאה לאור

1 במאי 2015

שם סדרה

Methods in Molecular Biology

לדלג לסוף של גלריית תמונות

לדלג להתחלה של גלריית תמונות

פרטים

This volume provides an overview of molecular biology tools for pathogen detection and characterization. Focusing on biobanking, biosafety, good laboratory practices, biological specimen collection and processing, quality assurance and control, validation of molecular diagnostic assays for veterinary use; molecular detection and identification of animal pathogens using a wide range of established techniques and covering, also, emerging diagnosis approaches; genotyping tools for assessing the genetic landscape and epidemiology of pathogens; and integrative omics and high-throughput technologies as powerful research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies aids scientists in continuing to study a broad range of molecular approaches, from simple and affordable to emerging and sophisticate.

מידע נוסף

מידע נוסף
מהדורה	2015 ed.
עמודים	536
פורמט	Hardback
ISBN10	1493920030
יצא לאור ב	New York, NY
תאריך יציאה לאור	1 במאי 2015
תוכן עניינים	PART I- Introducing Nucleic Acid Testing In The Veterinary Laboratory 1. Overview And Challenges Of Molecular Technologies In The Veterinary Microbiology Laboratory Monica V. Cunha And Joao Inacio 2. Significance And Integration Of Molecular Diagnostics in the Framework of Veterinary Practice Alicia Aranaz 3. Biosafety Principles And Practices for the Veterinary Diagnostic Laboratory Joseph Kozlovac And Beverly Schmitt 4. Veterinary Biobank Facility: Development And Management For Diagnostic and Research Purposes Tina Lombardo, Silvia Dotti, Riccardo Villa, Stefano Cinotti, And Maura Ferrari 5. Biological Specimen Collection And Processing for Molecular Analysis Susan Sanchez And Holly Sellers 6. Validation Of Molecular Diagnostic Assays And Quality Assurance and Control in the Veterinary Laboratory Nick Saunders and Ian Sharp PART II - Molecular Detection and Identification of Animal Pathogens in Laboratorial Settings 7. Molecular Approaches To Recognize Relevant And Emerging Infectious Diseases In Animals Fredrik Granberg, Oskar Karlsson, Mikael Leijon, Lihong Liu, and Sandor Belak 8. Real Time Reverse Transcriptase PCR For The Detection Of Bluetongue Virus Carrie Batten, Lorraine Frost, And Chris Oura 9. Nested And Multiplex Real Time PCR Using Dual-Labeled Probes: Detecting And Discriminating Mycobacterium Tuberculosis Complex Members In Cultures And Animal Tissues Pedro Costa, Isabel Couto, Miguel Viveiros, and Joao Inacio 10. A Real-Time PCR Assay For The Diagnosis Of Gastrointestinal Nematode Infections of Small Ruminants Florian Roeber, Aaron R. Jex, And Robin B. Gasser 11. Improved Detection Of Mycobacterium Bovis In Bovine Tissues Using Immunomagnetic Separation Approaches Irene R. Grant And Linda D. Stewart 12. Detection Of Fish Pathogens By Loop-Mediated Isothermal Amplification (LAMP) Technique Hatem Soliman, Mona Saleh, And Mansour El-Matbouli 13. Direct Detection Of Theileria Annulata In Bovine Blood Samples Using Standard And Isothermal DNA Amplification Approaches Jacinto Gomes And Joao Inacio 14. Reverse Line Blot Hybridization With Species-Specific Oligonucleotide Probes: Application To Piroplasms Detection Ana Hurtado 15. DNA Microarray-Based Detection Of Multiple Pathogens: Mycoplasma Spp. and Chlamydia Spp. Christiane Schnee and Konrad Sachse 16. In Situ Hybridization With Labeled Probes: Assessment of African Swine Fever Virus In Formalin-Fixed Paraffin-Embedded Tissues Maria Ballester and Fernando Rodriguez 17. Fluorescence In Situ Hybridization For The Tissue Detection of Bacterial Pathogens Associated With Porcine Infections Henrik Elvang Jensen, Louise Kruse Jensen, Kristiane Barington, Susanne Pors, Thomas Bjarnsholt, and Mette Boye 18. Identification Of Animal Pasteurellaceae By MALDI-TOF Mass Spectrometry Joachim Frey and Peter Kuhnert 19. Gold Nanoparticles as a Potential Tool For Diagnosis of Fish Diseases Mona Saleh, Hatem Soliman, and Mansour El-Matbouli 20. Nucleic Acid Testing, New Platforms And Nanotechnology For Point-of-Decision Diagnosis Of Animal Pathogens Fernando Teles And Luis Pina Fonseca PART III - Molecular Profiling Of Veterinary Relevant Microbial Pathogens 21. Molecular Typing Tools: From Pattern Recognition To Genome-Based Algorithms Konrad Sachse and Petra Moebius 22. Analysis Of Campylobacter Jejuni And Campylobacter Coli Genotypes In Poultry Flocks By Restriction Fragment Length Polymorphism (RFLP) Analysis Ana Claudia Carreira and Monica V. Cunha 23. Pulsed-Field Gel Electrophoresis (PFGE): Application In Population Structure Studies Of Bovine Mastitis-Causing Streptococci Ilda Santos-Sanches, Lelia Chambel, and Rogerio Tenreiro 24. Multiple-Locus Variable Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR And Multicolour Capillary Electrophoresis: Application To The Genotyping Of Brucella Species Giuliano Garofolo 25. Multilocus Sequence Typing (MLST): Markers For The Traceability Of Pathogenic Leptospira Strains Ahmed Ahmed, Ana S. Ferreira, and Rudy A. Hartskeerl 26. Single Nucleotide Polymorphisms Discrimination Using High-Resolution Melting Analysis For The Genotyping Of Bacillus Anthracis Sylviane Derzelle 27. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Analysis Of Members Of The Mycobacterium Tuberculosis Complex Ana Botelho, Ana Canto, Celia Leao, and Monica V. Cunha 28. Rapid Microarray-Based Genotyping Of Chlamydia Spp. Strains From Clinical Tissue Samples Konrad Sachse and Anke Ruettger 29. Multiplexed Genotyping Of Bacillus Anthracis By Luminex Xmap Suspension Array Simon Thierry and Sylviane Derzelle PART IV - Integrative Omics and High-Throughput Platforms to Unravel the Biology of Pathogens and Their Interaction With the Host 30. Next-Generation Sequencing In Veterinary Medicine: How Can The Massive Amount Of Information Arising From High-Throughput Technologies Improve Diagnosis, Control and Management Of Infectious Diseases? Steven Van Borm, Sandor Belak, Graham Freimanis, Alice Fusaro, Fredrik Granberg, Dirk Hoeper, Donald P. King, Isabella Monne, Richard Orton, and Toon Rosseel 31. Impact Of Next-Generation Technologies On Exploring Socio-Economically Important Parasites And Developing New Interventions Cinzia Cantacessi, Andreas Hofmann, Bronwyn E. Campbell, and Robin B. Gasser 32. Functional Genomics Of Tick Vectors Challenged With The Cattle Parasite Babesia Bigemina Ana Domingos, Sandra Antunes, Margarita Villar, and Jose de la Fuente33. Metagenomic Approaches To Disclose Disease-Associated Pathogens: Detection Of Viral Pathogens In Honeybees Fredrik Granberg, Oskar E. Karlsson, and Sandor Belak 34. Proteomics Characterization Of Tick-Host-Pathogen Interactions Marina Popara, Margarita Villar, and Jose de la Fuente